Xiaotang Lu

Aug 4, 2024 | nature.com | Xiaomeng Han |Xiaotang Lu |Peter Li |Zudi Lin |Daniel Berger |Yuelong Wu | +6 more

AbstractMapping neuronal networks is a central focus in neuroscience. While volume electron microscopy (vEM) can reveal the fine structure of neuronal networks (connectomics), it does not provide molecular information to identify cell types or functions. We developed an approach that uses fluorescent single-chain variable fragments (scFvs) to perform multiplexed detergent-free immunolabeling and volumetric-correlated-light-and-electron-microscopy on the same sample.

Sep 18, 2023 | biorxiv.org | Xiaotang Lu |Yuelong Wu |Peter Li |Tao Fang

AbstractDetergent-free immunolabeling has been proven feasible for correlated light and electron microscopy, but its application is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs.