Zudi Lin

Aug 4, 2024 | nature.com | Xiaomeng Han |Xiaotang Lu |Peter Li |Zudi Lin |Daniel Berger |Yuelong Wu | +6 more

AbstractMapping neuronal networks is a central focus in neuroscience. While volume electron microscopy (vEM) can reveal the fine structure of neuronal networks (connectomics), it does not provide molecular information to identify cell types or functions. We developed an approach that uses fluorescent single-chain variable fragments (scFvs) to perform multiplexed detergent-free immunolabeling and volumetric-correlated-light-and-electron-microscopy on the same sample.