Yanran Li

Dec 12, 2023 | mdpi.com | Yu Diao |Yaoxuan Zhang |Yanran Li |Jie Jiang

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Aug 18, 2023 | science.org | Xuan Zheng |Yanran Li |Jiarui Mi |Yu Zhao

AbstractPseudouridine is enriched in ribosomal, spliceosomal, transfer, and messenger RNA and thus integral to the central dogma. The chemical basis for how pseudouridine affects the molecular apparatus such as ribosome, however, remains elusive owing to the lack of structures without this natural modification. Here, we studied the translation of a hypopseudouridylated ribosome initiated by the internal ribosome entry site (IRES) elements.

Aug 18, 2023 | science.org | Xuan Zheng |Yanran Li |Jiarui Mi |Yajie Liu

Published In Science AdvancesVolume 9 | Issue 33August 2023Article versionsSubmission historyReceived: 16 January 2023Accepted: 19 July 2023PermissionsSee the Reprints and Permissions page for information about permissions for this article. AcknowledgmentsWe thank Clinical Laboratory of BGI Health (Shenzhen, China) for helping with mass spectrum analysis.

Aug 18, 2023 | science.org | Xuan Zheng |Yanran Li |Jiarui Mi |Ula V. Jurkunas

METHODSIn the preclinical phase, cadaveric donor corneas were obtained from the National Disease Research Interchange. Later in the clinical phase, patient limbal biopsies were obtained (surgical details below). For primary (P0) cell culture, a 3 mm–by–3 mm biopsy was harvested from the limbus, and dissected limbal epithelial sheets were digested with Liberase MNP-S (Roche, Basel, Switzerland) and then dissociated into a single-cell suspension.

Aug 18, 2023 | science.org | Xuan Zheng |Yanran Li |Jiarui Mi |Owen R. Liu

Published In Science AdvancesVolume 9 | Issue 33August 2023Article versionsSubmission historyReceived: 10 January 2023Accepted: 19 July 2023PermissionsSee the Reprints and Permissions page for information about permissions for this article. AcknowledgmentsFunding: This work was supported by David and Lucille Packard Foundation 2019-69817 (to O.R.L.) and NOAA Integrated Ecosystem Assessment Program.