Riza M. Daza

Sep 17, 2024 | nature.com | Florence M. Chardon |Riza M. Daza |Diego Calderon |Lea M. Starita |Nadav Ahituv

AbstractCRISPR-based gene activation (CRISPRa) is a strategy for upregulating gene expression by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe an experimental framework that combines highly multiplexed perturbations with single-cell RNA sequencing (sc-RNA-seq) to identify cell-type-specific, CRISPRa-responsive cis-regulatory elements and the gene(s) they regulate.

Feb 14, 2024 | nature.com | Chengxiang Qiu |Beth Martin |Riza M. Daza |Megan Taylor |Olivia Fulton |Xinxian Deng | +6 more

AbstractThe house mouse (Mus musculus) is an exceptional model system, combining genetic tractability with close evolutionary affinity to humans1,2. Mouse gestation lasts only 3 weeks, during which the genome orchestrates the astonishing transformation of a single-cell zygote into a free-living pup composed of more than 500 million cells.

Jan 23, 2024 | biorxiv.org | Sudarshan Pinglay |Jean-Benoît Lalanne |Riza M. Daza |Jonas Koeppel

AbstractThe functional consequences of structural variants (SVs) in mammalian genomes are challenging to study.