Byun J.A

Jun 11, 2024 | cell.com | Byun J.A |Declan R Creamer |Michael Smith Building |Oxford Road

Highlights•Regulation of PKA C subunits Tpk1–3 by the R subunit Bcy1 is isoform specific•Heat stress induces reversible Tpk3 sequestration and inactivation•The small heat shock protein Hsp42 is required for Tpk3 sequestration•Sequestration provides a non-canonical mechanism to fine-tune PKA signalingSummaryProtein kinase A (PKA) is a conserved kinase crucial for fundamental biological processes linked to growth, development, and metabolism.

Mar 26, 2024 | cell.com | Byrne D.P |Byun J.A |Julia Hardy

Time-lapse fluorescence imagingCells were washed twice with HBSS and then imaged in HBSS in the dark at room temperature. Forskolin (Fsk, Calbiochem), 3-isobutyl-1-methylxanthine (IBMX, Sigma), Isoproterenol (Iso, Sigma Aldrich), Propranolol (Prop, Sigma), Rapamycin (Rapa, LC Labs), and H89 (Cayman Chemical) were added as indicated.