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Jul 17, 2024 | 02089-1/fulltext&sz=64)
jbc.org | Byrne D.P |Chelsea Gary
AbstractProtein phosphorylation by kinases regulates mammalian cell functions, such as growth, division, and signal transduction. Among human kinases, NME1 and NME2 are associated with metastatic tumor suppression, but remain understudied due to the lack of tools to monitor their cellular substrates.
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Mar 27, 2024 | 01731-9/fulltext&sz=64)
jbc.org | Byrne D.P |Mingu Kang |Larner College |Amanda J. Senatore
ABSTRACTFocal adhesions (FAs) form the junction between extracellular matrix (ECM)-bound integrins and the actin cytoskeleton and also transmit signals that regulate cell adhesion, cytoskeletal dynamics, and cell migration. While many of these signals are rooted in reversible tyrosine phosphorylation, phosphorylation of FA proteins on Ser/Thr residues is far more abundant yet its mechanisms and consequences are far less understood.
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Mar 26, 2024 | 00184-9?rss=yes&sz=64)
cell.com | Byrne D.P |Byun J.A |Julia Hardy
Time-lapse fluorescence imagingCells were washed twice with HBSS and then imaged in HBSS in the dark at room temperature. Forskolin (Fsk, Calbiochem), 3-isobutyl-1-methylxanthine (IBMX, Sigma), Isoproterenol (Iso, Sigma Aldrich), Propranolol (Prop, Sigma), Rapamycin (Rapa, LC Labs), and H89 (Cayman Chemical) were added as indicated.
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Dec 28, 2023 | 00205-5/fulltext?fbclid=paaabyehkhg5vgpexkf9yjjqqxe3f7wfach2xmiomcwbcc7lmv7xcdb8_besa&sz=64)
arthroscopysportsmedicineandrehabilitation.org | Byrd J.W |Byrne D.P |Houston Methodist Orthopedics |Brendan M. Holderread
To evaluate online, self-reported pudendal nerve or perineal injuries related to the use of a perineal post during hip arthroscopy. Public posts on Reddit and the Health Organization for Pudendal Education were searched to identify anonymous individuals reporting symptoms of pudendal nerve or perineal injury following hip arthroscopy.
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Oct 31, 2023 | 01245-7&sz=64)
cell.com | Byrne D.P |Rebecca Gellman |Matthew R Olm |Steven K. Higginbottom
Highlights•Bacteroides and Prevotella sp. are isolated, sequenced from Hadza fecal samples•Bacteroides sp. encode more mucus degrading capacity than Prevotella sp. •Unlike Bacteroides, Prevotella colonization requires dietary plant fiber•Bacteroides outcompetes Prevotella in vivo on low plant fiber dietSummaryIndustrialization has transformed the gut microbiota, reducing the prevalence of Prevotella relative to Bacteroides.
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Sep 1, 2023 | 02242-1/abstract&sz=64)
jbc.org | Byrne D.P |Michael Smith Laboratories |Jacob F Wardman |Peter Rahfeld
ABSTRACTSulfation is widespread in Nature, and plays an important role in modulating biological function. Among the strategies developed by microbes to access sulfated oligosaccharides as a nutrient source is the production of 6-sulfoGlcNAcases to selectively release 6-sulfoGlcNAc from target oligosaccharides. Thus far, all 6-sulfoGlcNAcases identified have belonged to the large GH20 family of β-hexosaminidases.
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Aug 17, 2023 | 00056-2/fulltext&sz=64)
cartilagejournal.org | Byrne D.P |Zachary Stewart |F. Joseph Simeone |Ali Guermazi
1Volume fill of cartilage defect1. Complete filling or minor hypertrophy202. Major hypertrophy ≥150% or 75% to 99% filling of total defect volume153. 50% to 74% filling of total defect volume104. 25% to 49% filling of total defect volume55. <25% filling of total defect volume02Integration into adjacent cartilage1. Complete integration152. Split-like defect at repair tissue and native cartilage interface ≤2 mm103.
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Jun 13, 2023 | 00058-4/abstract&sz=64)
arthroplastytoday.org | Byrne D.P |Sayi P. Boddu |M. Lane Moore |Bryeson M. Rodgers
1Bargar WL, Bauer A, Börner M. Primary and revision total hip replacement using the Robodoc system. Clin Orthop Relat Res 1998:82–91. https://doi.org/10.1097/00003086-199809000-00011. •The Robodoc system is suggested to be a safe and effective tool for performing both primary and revision THAs.•Results indicate superior implant fit and positioning with less risk of femoral fractures. 2Cobb J, Henckel J, Gomes P, Harris S, Jakopec M, Rodriguez F, et al.
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Mar 6, 2023 | 00206-1&sz=64)
cell.com | Byrne D.P |Wenqiang Cao |Ines Sturmlechner
Antibodies Anti-human CD3 (HIT3a) BioLegend Cat# 300318, RRID:AB_314054 Anti-human CD3 (OKT3) BioLegend Cat# 317314, RRID:AB_571909 Anti-human CD4 (RPA-T4) BioLegend Cat# 300506, RRID:AB_314074 Anti-human CD8 (SK1) BioLegend Cat# 344723, RRID:AB_2562790 Anti-human CCR7 (G043H7) BioLegend Cat# 353207, RRID:AB_11203894 Anti-human CD25 (BC96) BioLegend Cat# 302635, RRID:AB_11219793 Anti-human CD122 (TU27) BioLegend Cat# 339017, RRID:AB_2721347 Anti-human IL2 (MQ1-17H12) BioLegend Cat# 500352,...
