Michael Smith Building

Sep 29, 2024 | bmcmethods.biomedcentral.com | Michael Smith Building

Efficient manipulation of recombinant DNA molecules is essential for the analysis of gene expression using transfection of reporter constructs into mammalian cell culture cells and to make transgenic organisms. Standard DNA ligase or Gibson assembly based cloning procedures are used for making smaller vectors such as plasmids, while manipulation of larger DNA molecules like bacterial artificial chromosomes (BACs) rely on homologous recombination in E.

Jun 11, 2024 | cell.com | Byun J.A |Declan R Creamer |Michael Smith Building |Oxford Road

Highlights•Regulation of PKA C subunits Tpk1–3 by the R subunit Bcy1 is isoform specific•Heat stress induces reversible Tpk3 sequestration and inactivation•The small heat shock protein Hsp42 is required for Tpk3 sequestration•Sequestration provides a non-canonical mechanism to fine-tune PKA signalingSummaryProtein kinase A (PKA) is a conserved kinase crucial for fundamental biological processes linked to growth, development, and metabolism.

Feb 29, 2024 | journals.plos.org | Zorana Carter |Michael Smith Building |Oxford Road |Declan R Creamer

Loading metrics Open Access Peer-reviewedResearch Article ? This is an uncorrected proof. Citation: Carter Z, Creamer D, Kouvidi A, Grant CM (2024) Sequestrase chaperones protect against oxidative stress-induced protein aggregation and [PSI+] prion formation. PLoS Genet 20(2): e1011194. https://doi.org/10.1371/journal.pgen.1011194Editor: Gregory P.

Sep 25, 2023 | cell.com | David Johnson |Michael Smith Building |Sun Yat-sen |David Burslem

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Mar 31, 2023 | onlinelibrary.wiley.com | Susanne Shultz |Michael Smith Building

The fallacy of the missing middle 1wIn the previous subsection, we pointed out that incorporating cognition into an analysis can result in a circular argument. The reason has to do with a derivative problem, the fallacy of the missing middle.