Claire Coupland

Jun 20, 2024 | science.org | Jaroslav Zak |Divij Mathew |Andrew E. Whiteley |Claire Coupland

AbstractIntercellular communication in the nervous system occurs through the release of neurotransmitters into the synaptic cleft between neurons. In the presynaptic neuron, the proton pumping vesicular- or vacuolar-type ATPase (V-ATPase) powers neurotransmitter loading into synaptic vesicles (SVs), with the V1 complex dissociating from the membrane region of the enzyme before exocytosis. We isolated SVs from rat brain using SidK, a V-ATPase-binding bacterial effector protein.

Dec 18, 2023 | biorxiv.org | T. Bertie Ansell |Megan Healy |Claire Coupland |Mark Sansom

AbstractMembrane Bound O-acyltransferases (MBOATs) are membrane embedded enzymes which catalyse acyl chain transfer to a diverse group of substrates including lipids, small-molecules and proteins. Recent MBOAT structures reveal a conserved structural core, despite wide-ranging functional specificity across both prokaryotes and eukaryotes.