David Hacker

Oct 26, 2024 | link.springer.com | David Hacker |Kelvin Lau |Florence Pojer |Laurence Durrer |Soraya Quinche |Ludovic Delisle

AbstractThe production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 106 cells/mL; and the subsequent addition of dimethyl sulfoxide at 2% (v/v).

Jun 26, 2023 | tandfonline.com | Christopher Jones |David Hacker |Carl R. Krynicki

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Mar 30, 2023 | foxnews.com | David Hacker

NEWYou can now listen to Fox News articles! No one expects to be fired when following the directives of their superiors. But that’s exactly what happened to Fire Chief Ron Hittle when the global leadership conference he attended was considered by the city of Stockton, California, to be a "religious event."After 24 years of protecting the lives and property of his community, Chief Hittle was fired not for disobeying orders but for his religious faith and activities.