Florence Pojer

Oct 26, 2024 | link.springer.com | David Hacker |Kelvin Lau |Florence Pojer |Laurence Durrer |Soraya Quinche |Ludovic Delisle

AbstractThe production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 106 cells/mL; and the subsequent addition of dimethyl sulfoxide at 2% (v/v).

May 28, 2024 | nature.com | Aaron S. Petruzzella |Marine Bruand |Albert Santamaria-Martínez |Natalya Katanayeva |Luc Reymond |Sandrine Georgeon | +6 more

AbstractCysteine cathepsins are a family of proteases that are relevant therapeutic targets for the treatment of different cancers and other diseases. However, no clinically approved drugs for these proteins exist, as their systemic inhibition can induce deleterious side effects. To address this problem, we developed a modular antibody-based platform for targeted drug delivery by conjugating non-natural peptide inhibitors (NNPIs) to antibodies.

May 17, 2023 | nature.com | Cristina Díaz-Perlas |Lluc Farrera-Soler |Florence Pojer |Kelvin Lau |Benjamin Ricken |Christian-Benedikt Gerhold

AbstractCommon inflammatory disorders such as ulcerative colitis and Crohn’s disease are non-invasively diagnosed or monitored by the biomarker calprotectin. However, current quantitative tests for calprotectin are antibody-based and vary depending on the type of antibody and assay used. Additionally, the binding epitopes of applied antibodies are not characterized by structures and for most antibodies it is unclear if they detect calprotectin dimer, tetramer, or both.