Soraya Quinche

Oct 26, 2024 | link.springer.com | David Hacker |Kelvin Lau |Florence Pojer |Laurence Durrer |Soraya Quinche |Ludovic Delisle

AbstractThe production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 106 cells/mL; and the subsequent addition of dimethyl sulfoxide at 2% (v/v).