Erik M Leith

Jul 16, 2024 | cell.com | Cheng Xu |Holly E. Kleinschmidt |Jianyu Yang |Erik M Leith

Highlights•Systematic dissection of genetic rules underlying FOXA1 binding specificity•FOXA1 binding is strongly promoted by co-binding TFs AP-1 and CEBPB•Chromatin context (e.g., heterochromatin) plays a minor role in FOXA1 binding•AP-1 is partially responsible for cell-type-specific binding of FOXA1SummaryDespite the unique ability of pioneer factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction of their genomic motifs.

Nov 11, 2023 | biorxiv.org | Cheng Xu |Holly E. Kleinschmidt |Jianyu Yang |Erik M Leith

AbstractDespite the unique ability of pioneer transcription factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction of their genomic motifs. The underlying mechanism of this selectivity is not well understood. Here, we design a high-throughput assay called ChIP-ISO to systematically dissect sequence features affecting the binding specificity of a classic PF, FOXA1.