Erik M Leith

4 weeks ago | biorxiv.org | Jiang Zhu |Erik M Leith |Erin O'Donnell |Bryan P. Manzano

AbstractBRD4, a bromodomain and extraterminal (BET) family transcriptional regulator of cell cycle progression, cell differentiation and cancer development, is believed to be recruited to chromatin via interactions between its tandem bromodomains (BD1 and BD2) and acetylated histone tails.

Jul 16, 2024 | cell.com | Cheng Xu |Holly E. Kleinschmidt |Jianyu Yang |Erik M Leith

Highlights•Systematic dissection of genetic rules underlying FOXA1 binding specificity•FOXA1 binding is strongly promoted by co-binding TFs AP-1 and CEBPB•Chromatin context (e.g., heterochromatin) plays a minor role in FOXA1 binding•AP-1 is partially responsible for cell-type-specific binding of FOXA1SummaryDespite the unique ability of pioneer factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction of their genomic motifs.

Nov 11, 2023 | biorxiv.org | Cheng Xu |Holly E. Kleinschmidt |Jianyu Yang |Erik M Leith

AbstractDespite the unique ability of pioneer transcription factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction of their genomic motifs. The underlying mechanism of this selectivity is not well understood. Here, we design a high-throughput assay called ChIP-ISO to systematically dissect sequence features affecting the binding specificity of a classic PF, FOXA1.