Eugene A. Katrukha

Feb 29, 2024 | nature.com | Dipti Rai |Victor Yin |Yixin Xu |Eugene A. Katrukha |Maarten A.F. Altelaar |Kai Jiang | +1 more

Abstractγ-Tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but the biochemical cross-talk between minus-end regulation pathways is poorly understood. Here we reconstituted this process in vitro using purified components. We found that all CAMSAPs could bind to the minus ends of γ-TuRC-attached microtubules.