Fiona Powrie

Oct 22, 2024 | nature.com | Maria Kiourlappou |Lisa Gartner |Nicholas E. Ilott |Fadi Issa |Joanna Hester |Jason Turner | +12 more

AbstractPrecision medicine in immune-mediated inflammatory diseases (IMIDs) requires a cellular understanding of treatment response. We describe a therapeutic atlas for Crohn’s disease (CD) and ulcerative colitis (UC) following adalimumab, an anti-tumour necrosis factor (anti-TNF) treatment. We generated ~1 million single-cell transcriptomes, organised into 109 cell states, from 216 gut biopsies (41 subjects), revealing disease-specific differences.

Apr 12, 2024 | nature.com | Luke S. Cox |Claire Pearson |Simon L. Priestnall |Anna Mikolajczak |James Briscoe |Fiona Powrie | +2 more

AbstractIntestinal immune responses to microbes are controlled by the cytokine IL-10 to avoid immune pathology. Here, we use single-cell RNA sequencing of colon lamina propria leukocytes (LPLs) along with RNA-seq and ATAC-seq of purified CD4+ T cells to show that the transcription factors Blimp-1 (encoded by Prdm1) and c-Maf co-dominantly regulate Il10 while negatively regulating proinflammatory cytokines in effector T cells.