Florence M. Chardon

Sep 17, 2024 | nature.com | Florence M. Chardon |Riza M. Daza |Diego Calderon |Lea M. Starita |Nadav Ahituv

AbstractCRISPR-based gene activation (CRISPRa) is a strategy for upregulating gene expression by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe an experimental framework that combines highly multiplexed perturbations with single-cell RNA sequencing (sc-RNA-seq) to identify cell-type-specific, CRISPRa-responsive cis-regulatory elements and the gene(s) they regulate.