Lea M. Starita

Sep 17, 2024 | nature.com | Florence M. Chardon |Riza M. Daza |Diego Calderon |Lea M. Starita |Nadav Ahituv

AbstractCRISPR-based gene activation (CRISPRa) is a strategy for upregulating gene expression by targeting promoters or enhancers in a tissue/cell-type specific manner. Here, we describe an experimental framework that combines highly multiplexed perturbations with single-cell RNA sequencing (sc-RNA-seq) to identify cell-type-specific, CRISPRa-responsive cis-regulatory elements and the gene(s) they regulate.

Jul 2, 2023 | genomebiology.biomedcentral.com | Douglas Fowler |David Adams |Anna L Gloyn |William Hahn |Debora S. Marks |James Neal | +5 more

Compiling a complete Atlas of Variant Effects for all 20,000 human genes, not to mention potentially hundreds of thousands of noncoding regulatory elements, will require an international collaborative effort involving thousands of researchers, clinicians and technologists. Comparing this initiative to some of the landmark genomic collaborative achievements of the past 30 years highlights some of the key challenges to be addressed.