Jeffrey M. Spraggins

Mar 1, 2024 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Correction to: Nature Cell Biology https://doi.org/10.1038/s41556-023-01194-w. Published online 19 July 2023. In the version of this article originally published, the name of Tianyang Xu was misspelled as Tiangyang Xu. The name has been corrected in the HTML and PDF versions of the article. About this articleJain, S., Pei, L., Spraggins, J.M. et al. Author Correction: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP). Nat Cell Biol (2024).

Jul 19, 2023 | nature.com | Ellen Quardokus |Diane Saunders |Elizabeth Zephyrine McDonough |John Hickey |Christopher Werlein |Hua Tian | +17 more

AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods.

Jul 19, 2023 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Data types, organs and technologiesSince the second quarter of 2023, HuBMAP datasets using 18 different analytical technologies or assay types have been publicly available (Figs. 1a and 2). These encompass 1,672 publicly available datasets from regions with no histopathologic abnormality from 14 organs across 180 donors. Protocols were optimized to minimize the ischaemia time of healthy donors. All published data are freely available via the HuBMAP portal (https://portal.hubmapconsortium.org).

Jul 19, 2023 | nature.com | Yashvardhan Jain |Leah L. Godwin |Yingnan Ju |Ellen Quardokus |Andreas Bueckle |Yiing Lin | +4 more

Data preparationFor the “HuBMAP – Hacking the Kidney” Kaggle competition, a dataset was compiled comprising 30 Periodic acid-Schiff (PAS) stain whole slide images (WSIs) with 7102 segmented renal glomeruli (see Supplementary Table 3 and “Methods”). To determine if algorithms generalize to other FTU types, a second dataset was compiled comprising seven colon hematoxylin and eosin (H&E) stain WSIs with 395 segmented colonic crypts (see Supplementary Table 4 and “Methods”).