Neil L. Kelleher

Dec 2, 2024 | nature.com | Rinako Nakagawa |Miriam Llorian |Neil L. Kelleher |Robert Klose |Martin R. Turner

AbstractTo increase antibody affinity against pathogens, positively selected GC-B cells initiate cell division in the light zone (LZ) of germinal centers (GCs). Among these, higher-affinity clones migrate to the dark zone (DZ) and vigorously proliferate by utilizing energy provided by oxidative phosphorylation (OXPHOS).

May 14, 2024 | nature.com | Tanja Habeck |Kyle Brown |Mowei Zhou |Michael Volný |Jeffrey N. Agar |Ying Ge | +4 more

AbstractThe combination of native electrospray ionization with top-down fragmentation in mass spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and cofactors. Although this approach is powerful, both native MS and top-down MS are not yet well standardized, and only a limited number of laboratories regularly carry out this type of research.

Mar 1, 2024 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Correction to: Nature Cell Biology https://doi.org/10.1038/s41556-023-01194-w. Published online 19 July 2023. In the version of this article originally published, the name of Tianyang Xu was misspelled as Tiangyang Xu. The name has been corrected in the HTML and PDF versions of the article. About this articleJain, S., Pei, L., Spraggins, J.M. et al. Author Correction: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP). Nat Cell Biol (2024).

Jul 19, 2023 | nature.com | Ellen Quardokus |Diane Saunders |Elizabeth Zephyrine McDonough |John Hickey |Christopher Werlein |Hua Tian | +17 more

AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods.

Jul 19, 2023 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Data types, organs and technologiesSince the second quarter of 2023, HuBMAP datasets using 18 different analytical technologies or assay types have been publicly available (Figs. 1a and 2). These encompass 1,672 publicly available datasets from regions with no histopathologic abnormality from 14 organs across 180 donors. Protocols were optimized to minimize the ischaemia time of healthy donors. All published data are freely available via the HuBMAP portal (https://portal.hubmapconsortium.org).