Justin Rendleman

Sep 6, 2024 | biorxiv.org | Justin Rendleman |Solomon A. Haizel |Shaohuan Wu |Xinye Ge

AbstractSequence elements within the 5' untranslated region (UTR) of eukaryotic genes, e.g. upstream open reading frames (uORFs), control translation of eukaryotic genes. We describe an element consisting of a start codon immediately followed by a stop codon which is distinct from uORFs in the lack of an elongation step. Start-stops have been described for specific cases, but their widespread impact has been overlooked.

Jul 13, 2024 | biorxiv.org | Jan Soroczynski |Lauren Anderson |Joanna Yeung |Justin Rendleman

AbstractTagmentation combines DNA fragmentation and sequencing adapter addition by leveraging the transposition activity of the bacterial cut-and-paste Tn5 transposase, to enable efficient sequencing library preparation. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5E54K, L372P protein per liter of E.

Jul 18, 2023 | biorxiv.org | Solomon A. Haizel |Shaohuan Wu |Justin Rendleman |Xinye Ge

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