Lauren Anderson

Jul 13, 2024 | biorxiv.org | Jan Soroczynski |Lauren Anderson |Joanna Yeung |Justin Rendleman

AbstractTagmentation combines DNA fragmentation and sequencing adapter addition by leveraging the transposition activity of the bacterial cut-and-paste Tn5 transposase, to enable efficient sequencing library preparation. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5E54K, L372P protein per liter of E.

Mar 25, 2024 | fresnobee.com | Lauren Anderson

Amazon’s first ever Big Spring Sale ends today, March 25. But there’s still time to grab some of the best deals in home, fashion, anti-aging beauty, and more! In addition to these great seasonal deals, Amazon offers customers fast and reliable delivery across a wide selection of products, and Prime members enjoy fast, free delivery on tens of millions of items as part of their membership.