Jan Soroczynski

Jul 13, 2024 | biorxiv.org | Jan Soroczynski |Lauren Anderson |Joanna Yeung |Justin Rendleman

AbstractTagmentation combines DNA fragmentation and sequencing adapter addition by leveraging the transposition activity of the bacterial cut-and-paste Tn5 transposase, to enable efficient sequencing library preparation. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5E54K, L372P protein per liter of E.

Sep 7, 2023 | jbc.org | Beverly Naigles |Jan Soroczynski

AbstractMacrophages must respond appropriately to pathogens and other pro-inflammatory stimuli in order to perform their roles in fighting infection. One way in which inflammatory stimuli can vary is in their dynamics – that is, the amplitude and duration of stimulus experienced by the cell. In this study, we performed long-term live cell imaging in a microfluidic device to investigate how the pro-inflammatory genes IRF1, CXCL10, and CXCL9 respond to dynamic interferon-gamma (IFNγ) stimulation.

May 22, 2023 | biorxiv.org | Avaneesh Narla |Lev Tsimring |Beverly Naigles |Jan Soroczynski

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