Joanna Yeung

Jul 13, 2024 | biorxiv.org | Jan Soroczynski |Lauren Anderson |Joanna Yeung |Justin Rendleman

AbstractTagmentation combines DNA fragmentation and sequencing adapter addition by leveraging the transposition activity of the bacterial cut-and-paste Tn5 transposase, to enable efficient sequencing library preparation. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5E54K, L372P protein per liter of E.

Dec 4, 2023 | biorxiv.org | Ishita Gupta |Joanna Yeung |Maryam Rahimi-Balaei |Sih-Rong Wu

AbstractIn the early cerebellar primordium, there are two progenitor zones, the ventricular zone (VZ) residing atop the IVth ventricle and the rhombic lip (RL) at the lateral edges of the developing cerebellum. These zones give rise to the several cell types that form the GABAergic and glutamatergic populations of the adult cerebellum, respectively. Recently, an understanding of the molecular compartmentation of these zones has emerged.

Jun 25, 2023 | bmcgenomics.biomedcentral.com | Joanna Yeung

Aug 8, 2022 | elifesciences.org | Joanna Yeung

Nov 17, 2018 | jove.com | Joanna Yeung