Lilach Sheiner

Jul 28, 2024 | nature.com | Shahar Bracha |Athena Economides |Daniel Walsh |Adriano Aguzzi |Lilach Sheiner

AbstractDelivering macromolecules across biological barriers such as the blood–brain barrier limits their application in vivo. Previous work has demonstrated that Toxoplasma gondii, a parasite that naturally travels from the human gut to the central nervous system (CNS), can deliver proteins to host cells. Here we engineered T.

May 25, 2024 | biorxiv.org | Fei Wu |Alexander W. Mühleip |Thomas E. Gruhl |Lilach Sheiner

AbstractRespiratory complexes have co-evolved into supercomplexes in different clades to sustain energy production at the basis of eukaryotic life. In parasites, they likely exhibit functional and compositional distinctions.

Jan 9, 2024 | biorxiv.org | Pradeep Kumar Sheokand |Alexander W. Mühleip |Lilach Sheiner

AbstractThe Plasmodium mitochondrial electron transport chain (mETC) is responsible for essential metabolic pathways such as de novo pyrimidine synthesis and ATP synthesis. The mETC complex III (cytochrome bc1 complex) is responsible for transferring electrons from ubiquinol to cytochrome c, and generating a proton gradient across the inner mitochondrial membrane, which is necessary for the function of ATP synthase.

Nov 1, 2023 | biorxiv.org | Lilach Sheiner |Amber L. Goerner

AbstractToxoplasmosis is a common protozoan infection that can have severe outcomes in the immunocompromised and during pregnancy, but treatment options are limited. Recently, nucleotide metabolism has received much attention as a target for new antiprotozoal agents and here we focus on pyrimidine salvage by Toxoplasma gondii as a drug target. Whereas uptake of [3H]-cytidine and particularly [3H]-thymidine was at most marginal, [3H]-uracil and [3H]-uridine were readily taken up.