Linus O Johannissen

Mar 4, 2024 | nature.com | Florence Hardy |Linus O Johannissen |Richard Obexer |Sam Hay

AbstractDirected evolution of computationally designed enzymes has provided new insights into the emergence of sophisticated catalytic sites in proteins. In this regard, we have recently shown that a histidine nucleophile and a flexible arginine can work in synergy to accelerate the Morita-Baylis-Hillman (MBH) reaction with unrivalled efficiency.

Aug 21, 2023 | nature.com | Derren J. Heyes |Muralidharan Shanmugam |Linus O Johannissen |Laura N Jeffreys |Sam Hay |Nigel S. Scrutton | +2 more

AbstractCarH is a coenzyme B12-dependent photoreceptor involved in regulating carotenoid biosynthesis. How light-triggered cleavage of the B12 Co-C bond culminates in CarH tetramer dissociation to initiate transcription remains unclear. Here, a series of crystal structures of the CarH B12-binding domain after illumination suggest formation of unforeseen intermediate states prior to tetramer dissociation.