Lorna Hopcroft

2 months ago | nature.com | Valentina Cutano |Eleanor M. Wigmore |Lorna Hopcroft |Stuart Williamson |Cath Eberlein |Simon T. Barry

AbstractLoss of PTEN expression, via homozygous or hemizygous deletion, is common in PIK3CA mutant ER + BC tumors. We assessed reduction of PTEN protein expression on AKT inhibitor capivasertib efficacy in PIK3CA altered tumors. In PIK3CA altered, PTEN protein high models, PI3Kα and AKT inhibition was effective, however ablation and partial PTEN expression reduction attenuated PI3Kαi but not AKTi efficacy, alone or combined with fulvestrant.

May 12, 2024 | nature.com | Thomas G. Hayhow |Rodrigo J. Carbajo |David Fisher |Frederick W. Goldberg |Lorna Hopcroft |Philip Hopcroft | +5 more

AbstractTargeting the estrogen receptor alpha (ERα) pathway is validated in the clinic as an effective means to treat ER+ breast cancers. Here we present the development of a VHL-targeting and orally bioavailable proteolysis-targeting chimera (PROTAC) degrader of ERα. In vitro studies with this PROTAC demonstrate excellent ERα degradation and ER antagonism in ER+ breast cancer cell lines. However, upon dosing the compound in vivo we observe an in vitro-in vivo disconnect.

Aug 18, 2023 | nature.com | Lorna Hopcroft |Stuart Williamson |Susana Ros |Larissa S. Carnevalli |Elza DeBruin

Correction to: npj Breast Cancer https://doi.org/10.1038/s41523-023-00571-w, published online 05 August 2023In this article the author name Scott Hoffmann, was incorrectly written as Scott Hoffman. The original article has been corrected.

Aug 5, 2023 | nature.com | Stuart Williamson |Susana Ros |Lorna Hopcroft |Larissa S. Carnevalli |Elza DeBruin

Cell culture and reagentsAll cell lines were authenticated using DNA fingerprinting short-tandem repeat (STR) assays. To generate resistant cell populations, parental cell lines were exposed to escalating concentrations of palbociclib over 4–6 months. T47D cells were cultured up to a final dose of 3 μM, which generated two PalboR cell pools (named T47D RB− & T47D CDK6H).