Luis F. Caldera

Jan 23, 2025 | cell.com | Eric Wang |Alexander Cohen |Luis F. Caldera |Jennifer R. Keeffe |Annie V. Rorick |Yusuf M. Adia | +3 more

Keywordsantibodycomputational methodsnanoparticleprotein designRBDsarbecovirusSARS-CoV-2vaccinationIntroductionEmerging SARS-CoV-2 variants, notably Omicron and its subvariants, have demonstrated the ability to partially evade previous vaccine-induced immune responses by mutating epitopes targeted by the generated antibodies,1,2,3,4,5,6,7,8,9 thus extending the duration and impact of the COVID-19 pandemic.

Feb 28, 2024 | biorxiv.org | Eric Wang |Alexander Cohen |Jennifer R. Keeffe |Luis F. Caldera

AbstractUsing computational methods, we designed 60-mer nanoparticles displaying SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs) by (i) creating RBD sequences with 6 mutations in the SARS-COV-2 WA1 RBD that were predicted to retain proper folding and abrogate antibody responses to variable epitopes (mosaic-2COMs; mosaic-5COM), and (ii) selecting 7 natural sarbecovirus RBDs (mosaic-7COM).