Jennifer R. Keeffe

Jan 23, 2025 | cell.com | Eric Wang |Alexander Cohen |Luis F. Caldera |Jennifer R. Keeffe |Annie V. Rorick |Yusuf M. Adia | +3 more

Keywordsantibodycomputational methodsnanoparticleprotein designRBDsarbecovirusSARS-CoV-2vaccinationIntroductionEmerging SARS-CoV-2 variants, notably Omicron and its subvariants, have demonstrated the ability to partially evade previous vaccine-induced immune responses by mutating epitopes targeted by the generated antibodies,1,2,3,4,5,6,7,8,9 thus extending the duration and impact of the COVID-19 pandemic.

Aug 26, 2024 | cell.com | Alexander Cohen |Jennifer R. Keeffe |Sandra E. Dross |Allison J Greaney

Highlights • Pan-sarbecovirus vaccine evaluated in pre-vaccinated monkeys and mice • Mosaic nanoparticles elicited broad antibody responses after COVID-19 vaccinations • Epitope and molecular fate mapping revealed cross-reactive recall responses • Original antigenic sin did not diminish immune responses to a mosaic vaccine Summary Immunization with mosaic-8b (nanoparticles presenting 8 SARS-like betacoronavirus [sarbecovirus] receptor-binding domains [RBDs]) elicits more broadly...

May 22, 2024 | biorxiv.org | Alexander Cohen |Jennifer R. Keeffe |Ariën Schiepers |Sandra E. Dross

AbstractImmunization with mosaic-8b [60-mer nanoparticles presenting 8 SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs)] elicits more broadly cross-reactive antibodies than homotypic SARS-CoV-2 RBD-only nanoparticles and protects against sarbecoviruses.

May 6, 2024 | nature.com | Rory A Hills |Tiong Kit Tan |Alexander Cohen |Jennifer R. Keeffe |Michelle Hill |Madeeha Afzal | +7 more

AbstractDefending against future pandemics requires vaccine platforms that protect across a range of related pathogens. Nanoscale patterning can be used to address this issue. Here, we produce quartets of linked receptor-binding domains (RBDs) from a panel of SARS-like betacoronaviruses, coupled to a computationally designed nanocage through SpyTag/SpyCatcher links.

Feb 28, 2024 | biorxiv.org | Eric Wang |Alexander Cohen |Jennifer R. Keeffe |Luis F. Caldera

AbstractUsing computational methods, we designed 60-mer nanoparticles displaying SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs) by (i) creating RBD sequences with 6 mutations in the SARS-COV-2 WA1 RBD that were predicted to retain proper folding and abrogate antibody responses to variable epitopes (mosaic-2COMs; mosaic-5COM), and (ii) selecting 7 natural sarbecovirus RBDs (mosaic-7COM).