Madison L. Doolittle

Jun 2, 2024 | nature.com | Vidyani Suryadevara |Adam D. Hudgins |Alberto Pappalardo |Alla Karpova |Amit K. Dey |Birgit Schilling | +23 more

AbstractOnce considered a tissue culture-specific phenomenon, cellular senescence has now been linked to various biological processes with both beneficial and detrimental roles in humans, rodents and other species. Much of our understanding of senescent cell biology still originates from tissue culture studies, where each cell in the culture is driven to an irreversible cell cycle arrest.

May 16, 2024 | jci.org | Dominik Saul |Madison L. Doolittle |Jennifer L. Rowsey |Mitchell N. Froemming

ResearchAgingBone biologyOpen Access | 10.1172/JCI179834 J Clin Invest. https://doi.org/10.1172/JCI179834. Copyright © 2024, Saul et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Feb 7, 2024 | biorxiv.org | Dominik Saul |Madison L. Doolittle |Jennifer L. Rowsey |Mitchell N. Froemming

AbstractCells expressing features of senescence, including upregulation of p21 and p16, appear transiently following tissue injury, yet the properties of these cells or how they contrast with age-induced senescent cells remains unclear. Here, we used skeletal injury as a model and identified the rapid appearance following fracture of p21+ cells expressing senescence markers, mainly as osteochondroprogenitors (OCHs) and neutrophils.

Jul 31, 2023 | nature.com | Madison L. Doolittle |Dominik Saul |Kevin D Pavelko |Joshua N Farr |David Monroe

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Apr 17, 2023 | jci.org | Joshua N Farr |Dominik Saul |Madison L. Doolittle |Japneet Kaur

AbstractClearance of senescent cells (SnCs) can prevent several age-related pathologies, including bone loss. However, the local versus systemic roles of SnCs in mediating tissue dysfunction remain unclear. Thus, we developed a mouse model (p16-LOX-ATTAC) that allowed for inducible SnC elimination (senolysis) in a cell-specific manner and compared the effects of local versus systemic senolysis during aging using bone as a prototype tissue.