Melanie Ott

Aug 28, 2024 | nature.com | Jae Kyu Ryu |Zhaoqi Yan |Mauricio Montano |Karuna Dixit |Rahul Suryawanshi |Yusuke Matsui | +10 more

AbstractLife-threatening thrombotic events and neurological symptoms are prevalent in COVID-19 and are persistent in patients with long COVID experiencing post-acute sequelae of SARS-CoV-2 infection1,2,3,4. Despite the clinical evidence1,5,6,7, the underlying mechanisms of coagulopathy in COVID-19 and its consequences in inflammation and neuropathology remain poorly understood and treatment options are insufficient.

Jul 3, 2024 | nature.com | Melanie Ott

AbstractAs SARS-CoV-2 continues to spread worldwide, tractable primary airway cell models that recapitulate the cell-intrinsic response to arising viral variants are needed. Here we describe an adult stem cell-derived human airway organoid model overexpressing the ACE2 receptor (ACE2-OE) that supports robust viral replication while maintaining 3D architecture and cellular diversity of the airway epithelium.

Oct 30, 2023 | biorxiv.org | Lauren Hall |Mir Khalid |Melanie Ott

AbstractColloidal aggregation is one of the largest contributors to false-positives in early drug discovery and chemical biology. Much work has focused on its impact on pure-protein screens; here we consider aggregations role in cell-based infectivity assays in Covid-19 drug repurposing. We began by investigating the potential aggregation of 41 drug candidates reported as SARs-CoV-2 entry inhibitors.

Sep 7, 2023 | cell.com | Steven Deeks |Melanie Ott

Co-immunoprecipitation (Co-IP) followed by western blottingApproximately 4 × 107 HEK 293T cells (from American Type Culture Collection) in two 150-mm dishes were transfected by plasmids (20 μg/dish) expressing FLAG-tagged BCL7C, KANSL2, or nothing. The following operations were all carried out at 4°C or on ice unless stated otherwise.

Jun 22, 2023 | jbc.org | Irene Chen |Melanie Ott

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