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Nov 28, 2024 | 00512-0?rss=yes&sz=64)
cell.com | Milica Moskovljevic |Filippo Dragoni |Nathan L. Board |Fengting Wu |Jun S. Lai |Hao Zhang | +10 more
Keywords HIV-1 persistence HIV-1 latent reservoir latency reversal antigenic stimulation dendritic cells CD4+ T cells cytomegalovirus Introduction Antiretroviral therapy (ART) effectively blocks viral replication, but HIV-1 persists in a small pool of latently infected resting CD4+ T cells harboring inducible, replication-competent proviruses.1,2,3 This reservoir was originally characterized with a quantitative viral outgrowth assay (QVOA) in which resting CD4+ T cells are stimulated with...
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Nov 18, 2024 | 
nature.com | Alton Barbehenn |Vivian Pae |Sannidhi Sarvadhavabhatla |Michael J Peluso |Jeffrey Martin |Frederick M. Hecht | +7 more
AbstractDespite antiretroviral therapy (ART), HIV persists in latently-infected cells (the HIV reservoir) which decay slowly over time. Here, leveraging >500 longitudinal samples from 67 people living with HIV (PLWH) treated during acute infection, we developed a mathematical model to predict reservoir decay from peripheral CD4 + T cells.
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Aug 28, 2024 | 
nature.com | Michael J Peluso |Michelle Davidson |Sarah Goldberg |Jessica Chen |Badri Viswanathan |Kevin C. Donohue | +8 more
AbstractTo understand the roles of acute-phase viral dynamics and host immune responses in post-acute sequelae of SARS-CoV-2 infection (PASC), we enrolled 136 participants within 5 days of their first positive SARS-CoV-2 real-time PCR test. Participants self-collected up to 21 nasal specimens within the first 28 days post-symptom onset; interviewer-administered questionnaires and blood samples were collected at enrollment, days 9, 14, 21, 28, and month 4 and 8 post-symptom onset.
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May 10, 2024 | 
pericles.pericles-prod.literatumonline.com | Yanhui Cai |Ondrej Podlaha |Steven Deeks |Cynthia Brinson
Conflict of interest YC, LZ, CRdV, EV, DSG, and JJW are employees and stockholders of Gilead Sciences, Inc. OP was an employee and a stockholder of Gilead Sciences, Inc., during the execution of this study. SGD declares research support from Gilead Sciences, Inc.
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May 10, 2024 | 
onlinelibrary.wiley.com | Yanhui Cai |Ondrej Podlaha |Steven Deeks |Cynthia Brinson
Conflict of interest YC, LZ, CRdV, EV, DSG, and JJW are employees and stockholders of Gilead Sciences, Inc. OP was an employee and a stockholder of Gilead Sciences, Inc., during the execution of this study. SGD declares research support from Gilead Sciences, Inc.
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Oct 2, 2023 | 
nature.com | Daniel Reeves |Mohamed Abdel-Mohsen |Mars Stone |Frederick M. Hecht |Jeffrey Martin |Steven Deeks | +2 more
AbstractPersistence of HIV in people living with HIV (PWH) on suppressive antiretroviral therapy (ART) has been linked to physiological mechanisms of CD4+ T cells. Here, in the same 37 male PWH on ART we measure longitudinal kinetics of HIV DNA and cell turnover rates in five CD4 cell subsets: naïve (TN), stem-cell- (TSCM), central- (TCM), transitional- (TTM), and effector-memory (TEM). HIV decreases in TTM and TEM but not in less-differentiated subsets.
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Sep 7, 2023 | 01066-5&sz=64)
cell.com | Steven Deeks |Melanie Ott
Co-immunoprecipitation (Co-IP) followed by western blottingApproximately 4 × 107 HEK 293T cells (from American Type Culture Collection) in two 150-mm dishes were transfected by plasmids (20 μg/dish) expressing FLAG-tagged BCL7C, KANSL2, or nothing. The following operations were all carried out at 4°C or on ice unless stated otherwise.
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Jul 19, 2023 | 
nature.com | Danillo G. Augusto |Lawton D. Murdolo |Noah D. Peyser |Victoria W. Murray |Cassandra Yun |Colin T. Maguire | +10 more
AbstractStudies have demonstrated that at least 20% of individuals infected with SARS-CoV-2 remain asymptomatic1,2,3,4. Although most global efforts have focused on severe illness in COVID-19, examining asymptomatic infection provides a unique opportunity to consider early immunological features that promote rapid viral clearance.
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Jul 13, 2023 | 
nature.com | Daniel Reeves |Thiago Oliveira |Michael J Peluso |Joshua T Schiffer |Steven Deeks
Human participantsThis study was approved by the institutional review board of the NIH National Institute of Allergy and Infectious Diseases and registered on ClinicalTrials.gov: NCT00039689. All study participants provided written informed consent, and leukapheresis products were collected in accordance with the protocol. All study participants were of male sex based on self-reporting. 5 participants were White, 3 Black and 2 Unknown/Hispanic-Latino.
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Jun 21, 2023 | 
nature.com | Raphael J Landovitz |Hyman Scott |Steven Deeks
Authors and AffiliationsCenter for Clinical AIDS Research and Education, David Geffen School of Medicine, University of California, Los Angeles, CA, USARaphael J. LandovitzBridge HIV, San Francisco Department of Public Health, San Francisco, CA, USAHyman ScottDivision of HIV, Infectious Diseases & Global Medicine, Department of Medicine, University of California, San Francisco, CA, USAHyman Scott & Steven G. DeeksContributionsS.G.D. researched data for the article.
