Mengrao Li

Jun 26, 2024 | pubs.rsc.org | Yan Ma |Mengrao Li |Yuzhong Zhang

Ratiometric electrochemical biosensor based on hybridization chain reaction signal amplification for sensitive microRNA-155 detection In this work, a sensitive ratiometric electrochemical biosensor for the microRNA-155 (miRNA-155) detection was reported based on hybridization chain reaction amplifying electrochemical signal.

Jun 23, 2023 | genomebiology.biomedcentral.com | Xin Zhang |Xinlong Wang |Jie Lv |Hongxin Huang |Jiahong Wang |Zhihong Tan | +9 more

Plasmid constructionFor U6+27 gRNA-expressing constructs, the DNA sequences including the hU6+27 promoter, a gRNA scaffold, and a gRNA insert site were synthesized and cloned into the pBluSKM vector. For Pre gRNA-expressing constructs, a second gRNA scaffold was inserted downstream of the gRNA insert site in the U6+27 gRNA-expressing constructs.

Apr 5, 2023 | pubs.rsc.org | Mengrao Li |Tingting Zhang |Yuzhong Zhang

Ultrasensitive electrochemical sensing platform for miRNA-21 detection based on manganese dioxide-gold nanoparticles nanoconjugates coupling with hybridization chain reaction and horseradish peroxidase signal amplification Mengrao Li,   Tingting Zhang  and  Yuzhong Zhang   Abstract Here, a ultrasensitive electrochemical miRNA-21 biosensor is described.