Raman van Wee

Dec 4, 2024 | biorxiv.org | Jiří Kratochvíl |Raman van Wee |Jan Christoph Thiele |Dan Loewenthal

AbstractMass photometry (MP) has emerged as a powerful approach to study biomolecular structure, dynamics and interactions. The capabilities of the method ultimately hinge on the ability to accurately measure the tiny optical contrast generated by individual molecules landing at a glass-water interface, which enables mass-resolved quantification of biomolecular mixtures.

Oct 22, 2024 | biorxiv.org | Raman van Wee |Yiwen Li |David Drechsel |Maria Popova

AbstractDNA loop extrusion by cohesin has emerged as a critical pathway for chromosome organization. In vitro single-molecule experiments indicate that loop extrusion requires the assembly of an heteropentameric complex consisting of the SMC1/SMC3 heterodimer, STAG1, NIPBL and the kleisin SCC1.

Feb 13, 2024 | nature.com | Mike Filius |Raman van Wee |Zeshi Li |sung kim |Martin Pabst |Dick de Ridder

AbstractProteins are the primary functional actors of the cell. While proteoform diversity is known to be highly biologically relevant, current protein analysis methods are of limited use for distinguishing proteoforms. Mass spectrometric methods, in particular, often provide only ambiguous information on post-translational modification sites, and sequences of co-existing modifications may not be resolved.

Sep 28, 2023 | biorxiv.org | Mike Filius |Carlos de Lannoy |Ilja Westerlaken |Raman van Wee

AbstractProteins are the primary functional actors of the cell. Hence, their identification is pivotal to advance our understanding of cell biology and disease. Current protein analysis methods are of limited use for distinguishing proteoforms. In particular, mass spectrometric methods often provide only ambiguous information on post-translational modification sites, and sequences of co-existing modifications may not be resolved.