Ronald T. Raines

Nov 11, 2024 | pubs.rsc.org | JoLynn B. Giancola |Ronald T. Raines

Endosomolytic Peptides Enable the Cellular Delivery of Peptide Nucleic Acids Precision genetic medicine enlists antisense oligonucleotides (ASOs) to bind to nucleic acid targets important for human disease. Peptide nucleic acids (PNAs) have many desirable attributes as ASOs but lack cellular permeability. Here, we use an assay based on the corrective splicing of an mRNA to assess the ability of synthetic peptides to deliver a functional PNA into a human cell.

Jun 18, 2024 | biorxiv.org | JoLynn B. Giancola |Ronald T. Raines

AbstractPrecision genetic medicine enlists antisense oligonucleotides (ASOs) to bind to nucleic acid targets important for human disease. Peptide nucleic acids (PNAs) have many desirable attributes as ASOs but lack cellular permeability. Here, we use an assay based on the corrective splicing of an mRNA to assess the ability of synthetic peptides to deliver a functional PNA into a human cell. We find that the endosomolytic peptides L17E and L17ER4 are highly efficacious delivery vehicles.

Jun 7, 2024 | onlinelibrary.wiley.com | Brian J. Levandowski |Brian Graham |Nile Abularrage |Ronald T. Raines

1 INTRODUCTION 5-Membered dienes such as 4H-pyrazoles are rapidly emerging as useful click reagents.1, 2 Spirocyclization provides an effective means to increase the Diels–Alder reactivity of 4H-pyrazoles by circumventing the disrupting effects of geminal repulsion in acyclic geminally substituted 5-membered dienes.3-6 Although 4H-pyrazoles containing five-membered spirocycles have been previously synthesized, they are predicted to be less reactive as Diels–Alder dienes than four-membered...

Sep 25, 2023 | pubs.acs.org | Brian J. Levandowski |Brian Graham |K. N. Houk |Ronald T. Raines

Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Org. Chem. 2024, XXXX, XXX Terms & Conditions Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses).

Sep 25, 2023 | dx.doi.org | Brian J. Levandowski |Brian Graham |K. N. Houk |Ronald T. Raines

Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Org. Chem. 2024, XXXX, XXX Terms & Conditions Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses).