Tarick J. El-Baba

Jan 13, 2025 | nature.com | Corinne A. Lutomski |Tarick J. El-Baba |Di Wu |Weston Struwe

AbstractUnderstanding the dynamics of membrane protein–ligand interactions within a native lipid bilayer is a major goal for drug discovery. Typically, cell-based assays are used, however, they are often blind to the effects of protein modifications. In this study, using the archetypal G protein-coupled receptor rhodopsin, we found that the receptor and its effectors can be released directly from retina rod disc membranes using infrared irradiation in a mass spectrometer.

Apr 10, 2024 | nature.com | Gang Du |Liam Healy |Liron David |Caitlin Walker |Tarick J. El-Baba |Corinne A. Lutomski | +12 more

AbstractGasdermin D (GSDMD) is the common effector for cytokine secretion and pyroptosis downstream of inflammasome activation and was previously shown to form large transmembrane pores upon cleavage by inflammatory caspases to generate the GSDMD N-terminal domain (GSDMD-NT)1-10. Here we report that GSDMD Cys191 is S-palmitoylated and palmitoylation is required for pore formation.