Thomas Naert

Oct 25, 2024 | nature.com | Fabiola Ceroni |Munevver B. Cicekdal |Nicolas Chassaing |Thomas Naert |Emma Kivuva |Soeren S Lienkamp | +5 more

AbstractAnophthalmia, microphthalmia and coloboma (AMC) comprise a spectrum of developmental eye disorders, accounting for approximately 20% of childhood visual impairment. While non-coding regulatory sequences are increasingly recognised as contributing to disease burden, characterising their impact on gene function and phenotype remains challenging. Furthermore, little is known of the nature and extent of their contribution to AMC phenotypes.

Sep 23, 2024 | biorxiv.org | Thomas Naert |Taiyo Yamamoto |Shuting Han |Melanie Horn

AbstractCRISPR-based genome engineering holds enormous promise for basic science and therapeutic applications. Integrating and editing DNA sequences is still challenging in many cellular contexts, largely due to insufficient control of the repair process. We find that repair at the genome-cargo interface is predictable by deep-learning models and adheres to sequence context specific rules.