Xuping Xie

Apr 8, 2024 | nature.com | Xuping Xie |Hongtao Yu |Jing Huang |Pei-Yong Shi

AbstractDrug resistance poses a significant challenge in the development of effective therapies against SARS-CoV-2. Here, we identified two double mutations, M49K/M165V and M49K/S301P, in the 3C-like protease (3CLpro) that confer resistance to a novel non-covalent inhibitor, WU-04, which is currently in phase III clinical trials (NCT06197217).

Jan 1, 2024 | nature.com | Jiakai Hou |Yanjun Wei |Long Sun |Shaoheng Liang |Ashley Guerrero |Xiaobo Mao | +7 more

AbstractHost anti-viral factors are essential for controlling SARS-CoV-2 infection but remain largely unknown due to the biases of previous large-scale studies toward pro-viral host factors. To fill in this knowledge gap, we perform a genome-wide CRISPR dropout screen and integrate analyses of the multi-omics data of the CRISPR screen, genome-wide association studies, single-cell RNA-Seq, and host-virus proteins or protein/RNA interactome.

Dec 10, 2023 | nature.com | Ching-Lin Hsieh |Andrew Horton |Xuping Xie |Hongjie Xia |Pei-Yong Shi |Andrew D Ellington | +7 more

AbstractThe ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has provided unprecedented insight into mutations enabling immune escape. Understanding how these mutations affect the dynamics of antibody-antigen interactions is crucial to the development of broadly protective antibodies and vaccines. Here we report the characterization of a potent neutralizing antibody (N3-1) identified from a COVID-19 patient during the first disease wave.

Oct 20, 2023 | nature.com | Jason Yeung |Pei-Yong Shi |Xuping Xie

AbstractAn attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 and 8 (∆3678) was previously reported to protect hamsters from SARS-CoV-2 infection and transmission. Here we report that a single-dose intranasal vaccination of ∆3678 protects K18-hACE2 mice from wild-type or variant SARS-CoV-2 challenge.