Jakob Nilsson

Nov 8, 2024 | nature.com | Arminja N. Kettenbach |Jakob Nilsson

AbstractPlk1 is a key mitotic kinase that localizes to distinct subcellular structures to promote accurate mitotic progression. Plk1 recruitment depends on direct interaction between polo-box domain (PBD) on Plk1 and PBD binding motif (PBD BM) on the interactors. However, recent study showed that PBD BM alone is not enough for stable binding between CENP-U and Plk1 highlighting the complexity of the interaction which warrants further investigation.

Sep 19, 2024 | nature.com | Ivo A. Hendriks |Norman E. Davey |Alessandra Zarantonello |Daniel R. Semlow |Shana J. Sturla |Michael Nielsen | +2 more

AbstractTranslesion DNA synthesis (TLS) is a cellular process that enables the bypass of DNA lesions encountered during DNA replication and is emerging as a primary target of chemotherapy. Among vertebrate DNA polymerases, polymerase κ (Polκ) has the distinctive ability to bypass minor groove DNA adducts in vitro. However, Polκ is also required for cells to overcome major groove DNA adducts but the basis of this requirement is unclear.

Jul 20, 2023 | nature.com | Yiqing Wang |Ivo A. Hendriks |Anna H. Bizard |Ronald T. Hay |Michael Nielsen |Jakob Nilsson | +1 more

AbstractSUMOylation regulates numerous cellular processes, but what represents the essential functions of this protein modification remains unclear. To address this, we performed genome-scale CRISPR–Cas9-based screens, revealing that the BLM-TOP3A-RMI1-RMI2 (BTRR)-PICH pathway, which resolves ultrafine anaphase DNA bridges (UFBs) arising from catenated DNA structures, and the poorly characterized protein NIP45/NFATC2IP become indispensable for cell proliferation when SUMOylation is inhibited.