Ronald T. Hay

Apr 22, 2024 | nature.com | Ivo A. Hendriks |Michael H. Tatham |Gian-luca McLelland |Ronald T. Hay |Michael Nielsen |Thijn R. Brummelkamp | +1 more

AbstractProtein SUMOylation provides a principal driving force for cellular stress responses, including DNA–protein crosslink (DPC) repair and arsenic-induced PML body degradation. In this study, using genome-scale screens, we identified the human E3 ligase TOPORS as a key effector of SUMO-dependent DPC resolution.

Jul 20, 2023 | nature.com | Yiqing Wang |Ivo A. Hendriks |Anna H. Bizard |Ronald T. Hay |Michael Nielsen |Jakob Nilsson | +1 more

AbstractSUMOylation regulates numerous cellular processes, but what represents the essential functions of this protein modification remains unclear. To address this, we performed genome-scale CRISPR–Cas9-based screens, revealing that the BLM-TOP3A-RMI1-RMI2 (BTRR)-PICH pathway, which resolves ultrafine anaphase DNA bridges (UFBs) arising from catenated DNA structures, and the poorly characterized protein NIP45/NFATC2IP become indispensable for cell proliferation when SUMOylation is inhibited.