Ivo A. Hendriks

Sep 19, 2024 | nature.com | Ivo A. Hendriks |Norman E. Davey |Alessandra Zarantonello |Daniel R. Semlow |Shana J. Sturla |Michael Nielsen | +2 more

AbstractTranslesion DNA synthesis (TLS) is a cellular process that enables the bypass of DNA lesions encountered during DNA replication and is emerging as a primary target of chemotherapy. Among vertebrate DNA polymerases, polymerase κ (Polκ) has the distinctive ability to bypass minor groove DNA adducts in vitro. However, Polκ is also required for cells to overcome major groove DNA adducts but the basis of this requirement is unclear.

Aug 4, 2024 | nature.com | Zita Fábián |Ellen S. Kakulidis |Ivo A. Hendriks |Ulrike Kühbacher |Nicolai B. Larsen |Marta Oliva-Santiago | +4 more

AbstractDNA-protein crosslinks (DPCs) are toxic lesions that inhibit DNA related processes. Post-translational modifications (PTMs), including SUMOylation and ubiquitylation, play a central role in DPC resolution, but whether other PTMs are also involved remains elusive. Here, we identify a DPC repair pathway orchestrated by poly-ADP-ribosylation (PARylation).

Apr 22, 2024 | nature.com | Ivo A. Hendriks |Michael H. Tatham |Gian-luca McLelland |Ronald T. Hay |Michael Nielsen |Thijn R. Brummelkamp | +1 more

AbstractProtein SUMOylation provides a principal driving force for cellular stress responses, including DNA–protein crosslink (DPC) repair and arsenic-induced PML body degradation. In this study, using genome-scale screens, we identified the human E3 ligase TOPORS as a key effector of SUMO-dependent DPC resolution.

Mar 18, 2024 | nature.com | Melanie Weisser |Andreas Mund |Blanca Lopez Mendez |Ivo A. Hendriks |Michael Nielsen |Hemmo Meyer | +2 more

AbstractThe hexameric AAA+ ATPase p97/VCP functions as an essential mediator of ubiquitin-dependent cellular processes, extracting ubiquitylated proteins from macromolecular complexes or membranes by catalyzing their unfolding. p97 is directed to ubiquitylated client proteins via multiple cofactors, most of which interact with the p97 N-domain.

Feb 6, 2024 | nature.com | Ivo A. Hendriks |Jonas Elsborg |Sara C. Buch-Larsen |Lene Terslev |Alexandra S. Rebak |Caroline Lennartsson

AbstractDespite the importance of citrullination in physiology and disease, global identification of citrullinated proteins, and the precise targeted sites, has remained challenging. Here we employed quantitative-mass-spectrometry-based proteomics to generate a comprehensive atlas of citrullination sites within the HL60 leukemia cell line following differentiation into neutrophil-like cells.