Juri Rappsilber

Sep 16, 2024 | pubs.acs.org | Zhuo Chen |Juri Rappsilber |Brian P Gibson |Flavio L. Schmidt

IntroductionClick to copy section linkSection link copied!The global brewing industry holds a significant position as a primary producer of alcoholic beverages, yielding an impressive 1.89 billion hectoliters in 2022. (1) However, this achievement comes with a notable environmental cost, marked by a substantial greenhouse gas (GHG) emission of approximately 30,172.15 kilotons of CO2 equivalent, with 22% originating from the supply chain, particularly raw materials.

May 14, 2024 | nature.com | Marcin Jaciuk |Alexander Rau |Alexander Hammermeister |Michał Rawski |Paulina Indyka |Urszula Jankowska | +7 more

AbstracttRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm5U34) in eukaryotic tRNAs. However, the structure and function of human Elongator remain poorly understood. In this study, we present a series of cryo-EM structures of human ELP123 in complex with tRNA and cofactors at four different stages of the reaction.

Nov 28, 2023 | biorxiv.org | Juri Rappsilber

AbstractCrosslinking mass spectrometry is a powerful tool for studying protein-protein interactions under native or near native conditions in complex mixtures. By help of novel search controls, we show that measures that aim to improve the number of identifications based on heuristic considerations can undermine error estimation in non-obvious ways. The relationship between decoys and false positives is very sensitive to the injection of information that favour likely correct matches.