Kevin Litchfield

Feb 8, 2025 | biorxiv.org | Kunal Shah |Paul Kennedy |James Black |Kevin Litchfield

AbstractLIMD1 is a tumour suppressor gene frequently lost in non-small cell lung cancer (NSCLC), but its role in cancer-immune cell interactions remains unexplored. Here, we demonstrate that LIMD1 loss results in upregulation of the key immune checkpoint protein PD-L1. Using multi-region sequencing from the TRACERx dataset, we identify that LIMD1 loss is clonal in over 80% of squamous cell carcinoma (LUSC) and 40% of lung adenocarcinoma (LUAD) cases, correlating with increased PD-L1 expression.

Jul 14, 2024 | nature.com | Richard Culliford |Charlie Mills |Alex J. Cornish |Ben Kinnersley |Amit Sud |Husayn Pallikonda | +5 more

AbstractClear cell renal cell carcinoma (ccRCC) is the most common form of kidney cancer, but a comprehensive description of its genomic landscape is lacking. We report the whole genome sequencing of 778 ccRCC patients enrolled in the 100,000 Genomes Project, providing for a detailed description of the somatic mutational landscape of ccRCC.

May 31, 2024 | nature.com | Robert Hynds |Ariana Huebner |David Pearce |Mark Hill |Ayse U. Akarca |David Moore | +17 more

AbstractPatient-derived xenograft (PDX) models are widely used in cancer research. To investigate the genomic fidelity of non-small cell lung cancer PDX models, we established 48 PDX models from 22 patients enrolled in the TRACERx study. Multi-region tumor sampling increased successful PDX engraftment and most models were histologically similar to their parent tumor.