Manolis Kellis

Oct 22, 2024 | nature.com | Lei Xiong |Manolis Kellis

AbstractGene expression involves transcription and translation. Despite large datasets and increasingly powerful methods devoted to calculating genetic variants’ effects on transcription, discrepancy between messenger RNA and protein levels hinders the systematic interpretation of the regulatory effects of disease-associated variants. Accurate models of the sequence determinants of translation are needed to close this gap and to interpret disease-associated variants that act on translation.

May 17, 2024 | nature.com | Brian D. Piening |Maedeh Mohebnasab |Bo Xia |Jeffrey S. Stern |Weimin Zhang |Jacqueline I. Kim | +20 more

AbstractIn a previous study, heart xenografts from 10-gene-edited pigs transplanted into two human decedents did not show evidence of acute-onset cellular- or antibody-mediated rejection. Here, to better understand the detailed molecular landscape following xenotransplantation, we carried out bulk and single-cell transcriptomics, lipidomics, proteomics and metabolomics on blood samples obtained from the transplanted decedents every 6 h, as well as histological and transcriptomic tissue profiling.