Mengyi Yang

Jan 10, 2025 | nature.com | Chang Li |Yi Xue |Jun Yao |Shan Sun |Mengyi Yang |Sen-fang Sui | +5 more

SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP. Here we use single-molecule fluorescence spectroscopy approaches to elucidate the chain of events that occur during the disassembly of SNARE complex by NSF. Our observations indicate two major pathways leading to the sequential disassembly of the SNARE complex: one where a syntaxin separated intermediate state is observed before syntaxin disassembles first, and a second where Vamp disassembles from the other proteins first. These studies uncover two parallel sequential pathways for the SNARE disassembly by NSF along with a syntaxin separated intermediate that couldn’t be observed otherwise. SNARE proteins are the minimal machinery required for the vesicle fusion in eukaryotes. Here the authors use single-molecule fluorescence spectroscopy to elucidate the chain of events during the disassembly of SNARE complex by NSF, showing two parallel sequential pathways for SNARE disassembly.