Jun Yao

Jan 10, 2025 | nature.com | Chang Li |Yi Xue |Jun Yao |Shan Sun |Mengyi Yang |Sen-fang Sui | +5 more

SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP. Here we use single-molecule fluorescence spectroscopy approaches to elucidate the chain of events that occur during the disassembly of SNARE complex by NSF. Our observations indicate two major pathways leading to the sequential disassembly of the SNARE complex: one where a syntaxin separated intermediate state is observed before syntaxin disassembles first, and a second where Vamp disassembles from the other proteins first. These studies uncover two parallel sequential pathways for the SNARE disassembly by NSF along with a syntaxin separated intermediate that couldn’t be observed otherwise. SNARE proteins are the minimal machinery required for the vesicle fusion in eukaryotes. Here the authors use single-molecule fluorescence spectroscopy to elucidate the chain of events during the disassembly of SNARE complex by NSF, showing two parallel sequential pathways for SNARE disassembly.

Dec 12, 2024 | mdpi.com | Jun Yao

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May 3, 2024 | nature.com | Jun Yao

AbstractOur study aimed to elucidate the molecular mechanisms underlying NAC1 (nucleus accumbens associated 1) transcriptional regulation of LDHA and its role in HBV immune evasion, thus contributing to the development of cirrhosis and hepatocellular carcinoma (HCC). Utilizing public datasets, we performed differential gene expression and weighted gene co-expression network analysis (WGCNA) on HBV-induced cirrhosis/HCC data.

Sep 3, 2023 | biorxiv.org | Georg Mohr |Jun Yao |Seung Park |Laura Markham

AbstractReverse transcriptase-Cas1 (RT-Cas1) fusion proteins found in some CRISPR systems enable spacer acquisition from both RNA and DNA, but the mechanism of RNA spacer acquisition has remained unclear.

Jul 21, 2023 | nature.com | Jun Yao

Organoid culture technology was named one of the "Top Ten Breakthroughs" by Science in 2013 and Nature Methods in 2017, and an excellent preclinical disease model by The New England Journal of Medicine in 2019 [63]. The organoid culture technology includes core technologies such as 3D culture, detection, and identification, as well as basic technologies such as stem cell isolation, organoid extraction, and organoid preservation [58, 64].