Shan Sun

Jan 10, 2025 | nature.com | Chang Li |Yi Xue |Jun Yao |Shan Sun |Mengyi Yang |Sen-fang Sui | +5 more

SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP. Here we use single-molecule fluorescence spectroscopy approaches to elucidate the chain of events that occur during the disassembly of SNARE complex by NSF. Our observations indicate two major pathways leading to the sequential disassembly of the SNARE complex: one where a syntaxin separated intermediate state is observed before syntaxin disassembles first, and a second where Vamp disassembles from the other proteins first. These studies uncover two parallel sequential pathways for the SNARE disassembly by NSF along with a syntaxin separated intermediate that couldn’t be observed otherwise. SNARE proteins are the minimal machinery required for the vesicle fusion in eukaryotes. Here the authors use single-molecule fluorescence spectroscopy to elucidate the chain of events during the disassembly of SNARE complex by NSF, showing two parallel sequential pathways for SNARE disassembly.

Oct 17, 2024 | bmjopen.bmj.com | Xiaoling Huang |Dongmei Tang |Shan Sun |Huawei Li

Statistics from Altmetric.com Request Permissions If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways. AudiologyNeurotologySLEEP MEDICINEThe study employs a combination of acceptance and commitment therapy (ACT) and tailored sound therapy to tackle insomnia associated with tinnitus.

Sep 23, 2023 | mdpi.com | Shan Sun |Younghwan Pan

1. IntroductionLeisure activities, such as fitness, are widely acknowledged to have positive impacts on individuals’ physical and mental health, as well as their mood []. Fitness centers have emerged as pivotal players in this scenario, evolving into a conventional service to fulfill public fitness requirements [].

Jul 26, 2023 | frontiersin.org | Chuang Liu |Jingjing Gao |Chuanjun Zhuo |Shan Sun

Jing Shen1† Qiang Wang1† Fengquan Lu1† Hua Xu1† Peng Wang2† Yu Feng1,3,4*† 1Medical laboratory, The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University, Suzhou, China 2Suzhou Key Laboratory of Neuro-Oncology and Nano-Bionics, Suzhou, China 3Medicine and Health, The University of New South Wales, Kensington, NSW, Australia 4Melbourne Medical School, The University of Melbourne, Parkville, VIC, Australia Background: The shortened life expectancy in schizophrenia (SCZ) patients may...