Chang Li

2 months ago | sciencedirect.com | Xianwei Fu |Shilong Jiao |Juan Li |Chang Li

Ammonia (NH3) is a crucial raw material for industrial products and holds significant potential for carbon-free energy storage and as a hydrogen carrier [1], [2]. Nowadays, the majority of industrial ammonia production relies on the Haber-Bosch process, which requires high temperatures, consumes large amounts of fossil fuels, and generates massive carbon dioxide emissions [3], [4].

Jan 10, 2025 | nature.com | Chang Li |Yi Xue |Jun Yao |Shan Sun |Mengyi Yang |Sen-fang Sui | +5 more

SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP. Here we use single-molecule fluorescence spectroscopy approaches to elucidate the chain of events that occur during the disassembly of SNARE complex by NSF. Our observations indicate two major pathways leading to the sequential disassembly of the SNARE complex: one where a syntaxin separated intermediate state is observed before syntaxin disassembles first, and a second where Vamp disassembles from the other proteins first. These studies uncover two parallel sequential pathways for the SNARE disassembly by NSF along with a syntaxin separated intermediate that couldn’t be observed otherwise. SNARE proteins are the minimal machinery required for the vesicle fusion in eukaryotes. Here the authors use single-molecule fluorescence spectroscopy to elucidate the chain of events during the disassembly of SNARE complex by NSF, showing two parallel sequential pathways for SNARE disassembly.

Nov 27, 2024 | mdpi.com | Chang Li |Fanhong Kong |Lei Feng |Han Sun

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Oct 30, 2024 | mdpi.com | Xing Han |Hao Zhan |Chang Li |Xuan Wang

All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.

Oct 24, 2024 | ceramics.onlinelibrary.wiley.com | Chang Li |Siyu Li |Pengfei Liu |Ping Tang

CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest. REFERENCES 1, . Computational simulation of liquid-fuelled HVOF thermal spraying. Surf Coat Technol. 2009; 204(5): 676–684. 2, , , . Computational fluid dynamic modeling of gas flow characteristics in a high-velocity oxy-fuel thermal spray system. J Therm Spray Technol. 2001; 10(3): 461–469. 3. The science and engineering of thermal spray coatings. Chichester, UK: John Wiley & Sons Ltd; 2008. 4, , .