Olexandr Dybkov

May 22, 2024 | nature.com | Zhenwei Zhang |VINAY KUMAR |Olexandr Dybkov |Henning Urlaub |Holger Stark

AbstractEarly spliceosome assembly can occur through an intron-defined pathway, whereby U1 and U2 small nuclear ribonucleoprotein particles (snRNPs) assemble across the intron1. Alternatively, it can occur through an exon-defined pathway2,3,4,5, whereby U2 binds the branch site located upstream of the defined exon and U1 snRNP interacts with the 5′ splice site located directly downstream of it.

Apr 3, 2024 | nature.com | Isaac Fianu |Moritz Ochmann |James Walshe |Olexandr Dybkov |Henning Urlaub

AbstractThe Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript1, but has not explained how Integrator removes Pol II from the DNA template.

Nov 6, 2023 | nature.com | Ying Chen |Olexandr Dybkov |Henning Urlaub

AbstractElongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB–ELOC subunit heterodimer.