Pablo Gainza

Oct 9, 2024 | biorxiv.org | Georg Petzold |Pablo Gainza |Stefano Annunziato |Ilaria Lamberto

AbstractThe CRL4CRBN ubiquitin ligase is leveraged by molecular glue degraders, small molecules that reprogram ligase specificity to induce degradation of clinically relevant neosubstrate proteins. Known CRBN neosubstrates share a generalizable β-hairpin G-loop recognition motif, yet systematic exploration of the CRBN target landscape is still pending.

Mar 4, 2024 | onlinelibrary.wiley.com | Yong Zhu |Lixia Wei |Pablo Gainza |Arnaud Charles-Antoine Zwygart

1 Introduction Influenza A virus (IAV) undergoes replication specifically within respiratory epithelial cells, and the infection is localized in the respiratory tracts. The initial stage of infection involves alveolar macrophages and monocytes, triggering a pro-inflammatory cytokine response.[1] The initiation of adaptive immunity relies on triggering dendritic cell (DC)[2] residing in the epithelia of the upper respiratory tract, where they acquire viral antigens.

Sep 22, 2023 | cell.com | Sanford Research |Pablo Gainza |Bruno E. Correia

Highlights•Systematic analysis of GPCR enzymatic activity unveils G protein selectivity across 124 GPCRs•Functional rank-order-based classification for GPCRs is introduced•Machine learning accurately predicts GPRC-Gα selectivity•Natural genetic variation in GPCRs influences their G protein preferencesSummaryG protein-coupled receptors (GPCRs) convert extracellular stimuli into intracellular signaling by coupling to heterotrimeric G proteins of four classes: Gi/o, Gq, Gs, and G12/13.

Aug 16, 2023 | annualreviews.org | Pablo Gainza |Vladas Oleinikovas |Thomas Ryckmans |Bernhard Fasching

INTRODUCTION Proteins levels in the cell are tightly regulated by the ubiquitin-proteasome system (UPS). Ubiquitin E3 ligases recognize specific structured and intrinsically unstructured elements (degrons) in the target protein, triggering their ubiquitination and degradation by the proteasome. This pathway can be rewired by small molecules, leading to targeted protein degradation of desired novel substrates, commonly referred as neosubstrates.

Aug 16, 2023 | annualreviews.org | Pablo Gainza |Vladas Oleinikovas |Thomas Ryckmans |Bernhard Fasching

INTRODUCTION Proteins levels in the cell are tightly regulated by the ubiquitin-proteasome system (UPS). Ubiquitin E3 ligases recognize specific structured and intrinsically unstructured elements (degrons) in the target protein, triggering their ubiquitination and degradation by the proteasome. This pathway can be rewired by small molecules, leading to targeted protein degradation of desired novel substrates, commonly referred as neosubstrates.