Raffael Schaffrath

Jan 17, 2025 | shorturl.at | Klaus Mayer |Raffael Schaffrath |Johannes Buchner |Lars Kaduhr

Abbreviations ACP, 3-amino-3-carboxypropyl group CCT/TriC, T-complex protein Ring Complex DPH1-8, protein/enzyme necessary for diphthamide synthesis 1–8 eEF2, eukaryotic translation elongation factor 2 HSP, heat shock protein Diphthamide on eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis [[1-3]]. Ribosomes that harbor eEF2 without diphthamide can generate mistranslated proteins due to higher incidences of −1 translational frameshifts [[4-6]].

May 14, 2024 | nature.com | Marcin Jaciuk |Alexander Rau |Alexander Hammermeister |Michał Rawski |Paulina Indyka |Urszula Jankowska | +7 more

AbstracttRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm5U34) in eukaryotic tRNAs. However, the structure and function of human Elongator remain poorly understood. In this study, we present a series of cryo-EM structures of human ELP123 in complex with tRNA and cofactors at four different stages of the reaction.