Rasmus O. Bak

Dec 15, 2024 | nature.com | Rasmus O. Bak

A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.

Mar 6, 2024 | nature.com | Bin Zhang |Rasmus O. Bak |Xin Gao |Nicolai J. Birkbak

AbstractMetastatic prostate cancer (PCa) poses a significant therapeutic challenge with high mortality rates. Utilizing CRISPR-Cas9 in vivo, we target five potential tumor suppressor genes (Pten, Trp53, Rb1, Stk11, and RnaseL) in the mouse prostate, reaching humane endpoint after eight weeks without metastasis. By further depleting three epigenetic factors (Kmt2c, Kmt2d, and Zbtb16), lung metastases are present in all mice.

Mar 6, 2024 | nature.com | Qi Wu |Rasmus O. Bak |Søren R. Paludan |Christoph F. Kollmann |Trine I. Jensen |Bao-cun Zhang

AbstractDNA is a danger signal sensed by cGAS to engage signaling through STING to activate innate immune functions. The best-studied downstream responses to STING activation include expression of type I interferon and inflammatory genes, but STING also activates other pathways, including apoptosis. Here, we report that STING-dependent induction of apoptosis in macrophages occurs through the intrinsic mitochondrial pathway and is mediated via IRF3 but acts independently of gene transcription.